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Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
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Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo
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May 22, 2011
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We present a lentiviral technique for genetic manipulation and visualization of single olfactory sensory neuron axon and its terminal arborization in vivo.
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Lentivirus-mediatedGenetic ManipulationVisualizationOlfactory Sensory NeuronsIn VivoOlfactory CircuitProjectionAxonsSynaptic TargetsOlfactory BulbMolecular MechanismsGene ExpressionVisualizingSingle OSN AxonsTerminal Arbor MorphologyLentiviral Based TechniqueMicroinjectionOlfactory EpitheliumExpression CassettesGenome IntegrationGreen Fluorescent Protein ExpressionReporter DetectionGene Function StudiesDevelopment PeriodOver-expressionShRNA Mediated Knock-downMorphologiesCell Bodies
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Sadrian, B., Chen, H., Gong, Q. Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo. J. Vis. Exp. (51), e2951, doi:10.3791/2951 (2011).
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