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Non-invasive Imaging of Leukocyte Homing and Migration in vivo
Chapters
- 00:05Title
- 00:55Introduction
- 01:25Animal Preparation
- 02:19Delivering an Inflammatory Stimulus to Induce Leukocyte Recruitment
- 03:19Imaging Stage Setup
- 04:17Video Acquisition
- 06:55Representative Time-Lapse Images
- 07:19Conclusion
Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
Tags
Non-invasive ImagingLeukocyte HomingLeukocyte MigrationIn Vivo ImagingTwo-photon MicroscopyHigh Resolution ImagingCell BehaviorLive MiceLeukocyte RecruitmentHost DefenseInfectionInflammatory DiseasesAutoimmune DiseasesIntravital ImagingSurgical PreparationBlood VesselsInflammationNon-invasive Single-cell ImagingLeukocyte TraffickingMouse Footpad And Phalanges
