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Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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07:42 min
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December 05, 2010
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Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.
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Non-invasive ImagingLeukocyte HomingLeukocyte MigrationIn Vivo ImagingTwo-photon MicroscopyHigh Resolution ImagingCell BehaviorLive MiceLeukocyte RecruitmentHost DefenseInfectionInflammatory DiseasesAutoimmune DiseasesIntravital ImagingSurgical PreparationBlood VesselsInflammationNon-invasive Single-cell ImagingLeukocyte TraffickingMouse Footpad And Phalanges
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Wang, B., Zinselmeyer, B. H., McDole, J. R., Gieselman, P. A., Miller, M. J. Non-invasive Imaging of Leukocyte Homing and Migration in vivo. J. Vis. Exp. (46), e2062, doi:10.3791/2062 (2010).
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