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Non-invasive Imaging of Leukocyte Homing and Migration in vivo
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JoVE 杂志 免疫与感染
Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Non-invasive Imaging of Leukocyte Homing and Migration in vivo

DOI:
10.3791/2062-v

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07:42 min

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December 05, 2010

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, , , ,
1Department of Pathology and Immunology,Washington University in St. Louis, 2National Institute of Neurological Disorders and Stroke, NINDS,NIH – National Institute of Health

Chapters

  • 00:05Title
  • 00:55Introduction
  • 01:25Animal Preparation
  • 02:19Delivering an Inflammatory Stimulus to Induce Leukocyte Recruitment
  • 03:19Imaging Stage Setup
  • 04:17Video Acquisition
  • 06:55Representative Time-Lapse Images
  • 07:19Conclusion

Summary

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Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.

Tags

Non-invasive ImagingLeukocyte HomingLeukocyte MigrationIn Vivo ImagingTwo-photon MicroscopyHigh Resolution ImagingCell BehaviorLive MiceLeukocyte RecruitmentHost DefenseInfectionInflammatory DiseasesAutoimmune DiseasesIntravital ImagingSurgical PreparationBlood VesselsInflammationNon-invasive Single-cell ImagingLeukocyte TraffickingMouse Footpad And Phalanges

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