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Fixation of Embryonic Mouse Tissue for Cytoneme Analysis
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

English

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2,116 Views

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08:46 min

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June 16, 2022

DOI:

10.3791/64100-v

DOI:
10.3791/64100-v

•

08:46 min
June 16, 2022

•

8 Views
, , , ,
1Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, 2St. Jude Graduate School of Biomedical Sciences

Summary

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Here, an optimized step-by-step protocol is provided for fixation, immunostaining, and sectioning of embryos to detect specialized signaling filopodia called cytonemes in developing mouse tissues.

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Cite this Article

Hall, E. T., Daly, C. A., Zhang, Y., Dillard, M. E., Ogden, S. K. Fixation of Embryonic Mouse Tissue for Cytoneme Analysis. J. Vis. Exp. (184), e64100, doi:10.3791/64100 (2022).

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