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Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
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Neuroscience
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JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

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3,130 Views

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08:18 min

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April 16, 2021

DOI:

10.3791/62405-v

DOI:
10.3791/62405-v

•

08:18 min
April 16, 2021

•

7 Views
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1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science,Fudan University, 4Department of Psychiatry,Icahn School of Medicine at Mount Sinai

Summary

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We have developed a method that enriches for and isolates human astrocyte populations from fresh-frozen tissue for use in downstream molecular analyses.

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Mussa, Z., Tome-Garcia, J., Jiang, Y., Akbarian, S., Tsankova, N. M. Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting. J. Vis. Exp. (170), e62405, doi:10.3791/62405 (2021).

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