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Utilizando um dispositivo microfluídica para estimulação mecânica e alta resolução de imagem de c. elegans
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JoVE Journal 神経科学
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Utilizando um dispositivo microfluídica para estimulação mecânica e alta resolução de imagem de c. elegans

9,583 Views

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10:39 min

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February 19, 2018

DOI:

10.3791/56530-v

DOI:
10.3791/56530-v

•

10:39 min
February 19, 2018

•

1 Views
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1Department of Molecular and Cellular Physiology,Stanford University, 2機械工学科,Stanford University, 3生物工学科,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

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微小流体力学Mechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

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Cite this Article

Fehlauer, H., Nekimken, A. L., Kim, A. A., Pruitt, B. L., Goodman, M. B., Krieg, M. Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans. J. Vis. Exp. (132), e56530, doi:10.3791/56530 (2018).

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