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重组DNA和蛋白质复合物，用原子力显微镜的可视化

JoVE Journal
生物工学

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JoVE Journal 生物工学

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

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重组DNA和蛋白质复合物，用原子力显微镜的可视化

22,114 Views

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08:30 min

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July 18, 2011

DOI:

DOI:

•

08:30 min

July 18, 2011

•

9 Views

Patrick J. M. Murphy¹, Morgan Shannon², John Goertz²

¹College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, ²College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

概要

是描述一个轻敲模式原子力显微镜（AFM）的可视化的质粒DNA，细胞质的蛋白质和DNA -蛋白质复合物的方法。该方法包括准备样品的原子力显微镜成像，生化操纵的替代方法。在近生理缓冲条件下观察DNA含有特定的蛋白相互作用的区域。

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