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Isolierung von aktivem Caenorhabditis elegans Kernextrakt und Rekonstitution für in vitro Transkription
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Biochimica
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JoVE Journal Biochimica
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
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Isolierung von aktivem Caenorhabditis elegans Kernextrakt und Rekonstitution für in vitro Transkription

2,345 Views

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06:09 min

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August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

7 Views
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1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Summary

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Hier beschreiben wir ein detailliertes Protokoll zur Isolierung von aktivem Kernextrakt aus Larvenstadium 4 C. elegans und visualisierung der Transkriptionsaktivität in einem in vitro System.

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Cite this Article

Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

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