Rivista
/
Immunologia e infezioni
/
Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

6,611 Views

•

07:54 min

•

December 21, 2019

DOI:

10.3791/60272-v

DOI:
10.3791/60272-v

•

07:54 min
December 21, 2019

•

1 Views
, , , ,
1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging,University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery,University Hospitals and University of Geneva

Summary

Automatically generated

This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

Explore More Videos

Orthotopic ModelPleural MesotheliomaAthymic MicePET/CTImplantationMonitoringAnesthesiaBuprenorphinePovidone-iodineCell SuspensionHamilton SyringeAbsorbable SuturesFDGDose CalculatorRadioactivity

Read Article

Cite this Article

Colin, D. J., Bejuy, O., Germain, S., Triponez, F., Serre-Beinier, V. Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice. J. Vis. Exp. (154), e60272, doi:10.3791/60272 (2019).

Download .ris file

Copy

Share Video

.

Copy