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Immunologie et infection
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Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
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Immunologie et infection
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Journal JoVE Immunologie et infection
Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

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07:54 min

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December 21, 2019

DOI:

10.3791/60272-v

DOI:
10.3791/60272-v

•

07:54 min
December 21, 2019

•

1 Views
, , , ,
1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging,University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery,University Hospitals and University of Geneva

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This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

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Orthotopic ModelPleural MesotheliomaAthymic MicePET/CTImplantationMonitoringAnesthesiaBuprenorphinePovidone-iodineCell SuspensionHamilton SyringeAbsorbable SuturesFDGDose CalculatorRadioactivity

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Colin, D. J., Bejuy, O., Germain, S., Triponez, F., Serre-Beinier, V. Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice. J. Vis. Exp. (154), e60272, doi:10.3791/60272 (2019).

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