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Immunology and Infection

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JoVE Journal Immunology and Infection
Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice
 

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

Article DOI: 10.3791/60272-v • 07:54 min • December 21st, 2019
December 21st, 2019 •
1, 1, 1, 2, 2
1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging, University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva

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This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

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Keywords: Orthotopic Model Pleural Mesothelioma Athymic Mice PET/CT Implantation Monitoring Anesthesia Buprenorphine Povidone-iodine Cell Suspension Hamilton Syringe Absorbable Sutures FDG Dose Calculator Radioactivity
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