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Biochemistry

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JoVE Journal Biochemistry
LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
 

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

Article DOI: 10.3791/55626-v • 08:01 min • April 9th, 2017
April 9th, 2017 •
*1, *1, 1
1Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University
* These authors contributed equally

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Here we present a step-by-step protocol of the long-length electrostatic repulsion-hydrophilic interaction chromatography-tandem mass spectrometry (LERLIC-MS/MS) method. This is a novel methodology that enables for the first time quantification and characterization of the glutamine and asparagine deamidation isoforms by shotgun proteomics.

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