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Enhancing EV Isolation for Precise Biofluid Analysis

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Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis (Video) | JoVE

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Biochimie

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Journal JoVE Biochimie

Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis

Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis (Video) | JoVE

Enhancing EV Isolation for Precise Biofluid Analysis

DOI:

10.3791/200750-v

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02:56 min

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October 27, 2023

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Yi-Kai Liu, Zhuojun Luo, Anton Iliuk², Weiguo Andy Tao^2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

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Keywords: Extracellular Vesicles (EVs)Proteomics Phosphoproteomics Sample Preparation Lysis Buffer Trypsin Digestion Desalting Phosphopeptide Enrichment

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