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LERLIC-MS / MS进行深入表征和谷氨酰胺的定量和天冬酰胺脱酰胺在鸟枪蛋白质组学

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Biochimie

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Journal JoVE Biochimie

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

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LERLIC-MS / MS进行深入表征和谷氨酰胺的定量和天冬酰胺脱酰胺在鸟枪蛋白质组学

7,983 Views

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08:01 min

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April 09, 2017

DOI:

10.3791/55626-v

DOI:

10.3791/55626-v

•

08:01 min

April 09, 2017

•

5 Views

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze¹

¹Division of Structural Biology and Biochemistry, School of Biological Sciences,Nanyang Technological University

Summary

这里，我们提出的长条静电排斥亲水相互作用色谱 - 串联质谱（LERLIC-MS / MS）的方法的一个步骤一步协议。这是一种新的方法，使通过鸟枪蛋白质组学的谷氨酰胺和天冬酰胺脱酰胺亚型的第一次定量和定性。

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