/

/

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

/

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution (Video) | JoVE

Journal JoVE
Biochimie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biochimie

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution (Video) | JoVE

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:

10.3791/65236-v

•

01:31 min

•

May 05, 2023

•

Lukasz S. Borowski², Karolina Kasztelan³, Jolanta Czerwinska-Kostrzewska², Roman J. Szczesny

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Tags

Keywords: Mitochondrial DNA DNA Synthesis DNA Distribution High-throughput Image-based Quantification Mitochondrial Replication Mitochondrial Maintenance Interferon Response Pathway Oxidative Phosphorylation 5-bromo-2′-deoxyuridine (BrdU) Incorporation Immunofluorescence Detection

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution (Video) | JoVE

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis (Video) | JoVE

Cell Transfection and 5-Bromo-2'-Deoxyuridine (BrdU) Incorporation in HeLa Cells (Video) | JoVE

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE

HeLa Cell Fixation with Hoechst 33342 and Blocking (Video) | JoVE

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

Read Article