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Bioluminescentie beeldvorming van NADPH-oxidase activiteit in verschillende diermodellen
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Immunologie et infection
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Journal JoVE Immunologie et infection
Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
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Bioluminescentie beeldvorming van NADPH-oxidase activiteit in verschillende diermodellen

16,065 Views

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10:42 min

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October 22, 2012

DOI:

10.3791/3925-v

DOI:
10.3791/3925-v

•

10:42 min
October 22, 2012

•

4 Views
, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Departments of Medicine and Immunology,Roswell Park Cancer Institute, 3Department of Medicine,University at Buffalo School of Medicine

Summary

Automatically generated

NADPH oxidase is de belangrijkste bron van reactieve zuurstof species (ROS) in fagocyten. Vanwege de vluchtigheid van ROS is moeilijk te meten en te controleren ROS levels in levende dieren. Een minimaal invasieve methode voor seriële kwantificering van ROS in levende muizen beschreven.

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Cite this Article

Han, W., Li, H., Segal, B. H., Blackwell, T. S. Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models. J. Vis. Exp. (68), e3925, doi:10.3791/3925 (2012).

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