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Analyse av Cardiac kammer utvikling under musen Embryogenesis bruke hele Mount Epifluorescence
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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Analyse av Cardiac kammer utvikling under musen Embryogenesis bruke hele Mount Epifluorescence

7,295 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
,
1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

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Vi presenterer protokoller for å undersøke musen heart utvikling med hele mount epifluorescent mikroskopi på mouse embryoer dissekert fra ventrikkel bestemt MLC-2v-tdTomato reporter banke i mus. Denne metoden tillater oss å visualisere direkte hvert trinn av ventrikkel formasjonen under musen heart utvikling uten arbeidskrevende histochemical metoder.

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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