Here, we present a standardized series of protocols to observe the superior ocular sulcus, a recently-identified, evolutionarily-conserved structure in the vertebrate eye. Using zebrafish larvae, we demonstrate techniques necessary to identify factors that contribute to the formation and closure of the superior ocular sulcus.
Yoon, K. H., Widen, S. A., Wilson, M. M., Hocking, J. C., Waskiewicz, A. J. Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis. J. Vis. Exp. (145), e59259, doi:10.3791/59259 (2019).