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Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
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Chapters
- 00:05Title
- 01:03Snap-freezing Embryonic Mouse Hearts
- 02:02Paraformaldehyde Fixing, Cryoprotecting, and OCT Embedding
- 02:55Cryosectioning
- 03:51Immunofluorescence
- 05:16Confocal Imaging and Image Analysis
- 08:30Results: Analysis of Cardiomyocyte Development
- 10:27Conclusion
Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.
Tags
Keywords: CardiomyocyteHeart DevelopmentImmunofluorescenceEmbryonic Mouse HeartSarcomeresMyofibrilsIntercalated DiscsCostameresMyocardial DevelopmentStructural And Functional UnitsDevelopmental Heart DefectsCultured CardiomyocytesIn Vivo Mechanical InputsEndocardial CuesSensitivityResolutionCo-immunostainingFluorescent ProteinStructural ChangesMutationsDevelopmental Heart Defects
