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Analys av hjärtats kammare utveckling under musen embryogenes använder hela Mount Epifluorescence
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Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
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Analys av hjärtats kammare utveckling under musen embryogenes använder hela Mount Epifluorescence

7,295 Views

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06:27 min

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April 17, 2019

DOI:

10.3791/59413-v

DOI:
10.3791/59413-v

•

06:27 min
April 17, 2019

•

9 Views
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1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Vanderbilt Center for Stem Cell Biology,Vanderbilt University

Summary

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Vi presenterar de protokoll för att undersöka mus hjärtat utveckling med hela mount epifluorescerande mikroskopi på musembryon dissekeras från ventrikulära specifika MLC-2v-tdTomato reporter inpressning möss. Denna metod tillåter oss att direkt visualisera varje skede av ventrikulära bildandet under musen hjärtat utveckling utan arbetsintensiva histochemical metoder.

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Cardiac Chamber DevelopmentMouse EmbryogenesisWhole Mount EpifluorescenceMLC-2v-tdTomatoHeart Tube FormationLoopingChamber FormationFluorescent ReporterDissectionGenotypingEpifluorescent Imaging

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Cite this Article

Zhang, Z., Nam, Y. Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence. J. Vis. Exp. (146), e59413, doi:10.3791/59413 (2019).

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