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Isolamento da Caenorhabditis Ativa elegans Extrato Nuclear e Reconstituição para Transcrição In Vitro
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Biochemie
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JoVE Journal Biochemie
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Isolamento da Caenorhabditis Ativa elegans Extrato Nuclear e Reconstituição para Transcrição In Vitro

2,340 Views

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06:09 min

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August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

7 Views
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1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Summary

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Aqui, descrevemos um protocolo detalhado para isolar o extrato nuclear ativo do estágio 4 C. elegans larval e visualizar a atividade de transcrição em um sistema in vitro .

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Cite this Article

Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

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