Journal
/
Biochemie
/
생체외 전사에 대한 활성 Caenorhabditis elegans 핵 추출물 및 재구성의 고립
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
Please note that all translations are automatically generated. Click here for the English version.

생체외 전사에 대한 활성 Caenorhabditis elegans 핵 추출물 및 재구성의 고립

2,318 Views

•

06:09 min

•

August 11, 2021

DOI:

10.3791/62723-v

DOI:
10.3791/62723-v

•

06:09 min
August 11, 2021

•

5 Views
,
1Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Tags

Caenorhabditis ElegansNuclear ExtractIn Vitro TranscriptionBalch HomogenizerHypotonic BufferHypertonic BufferSynchronizationL1 AnimalsL4 StageOP50CentrifugationHomogenizationTungsten Carbide Ball

Read Article

Cite this Article

Wibisono, P., Sun, J. Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription. J. Vis. Exp. (174), e62723, doi:10.3791/62723 (2021).

Download .ris file

Copy

Share Video

.

Copy