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分离培养成年斑马鱼脑源性神经球
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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分离培养成年斑马鱼脑源性神经球

12,607 Views

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11:01 min

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February 29, 2016

DOI:

10.3791/53617-v

DOI:
10.3791/53617-v

•

11:01 min
February 29, 2016

•

8 Views
, , , ,
1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Summary

Automatically generated

在这里，我们提供一个可重复的方法，使用从整个脑或从任一端脑，顶盖或成年斑马鱼脑的小脑区域衍生的神经球测定以检查成人神经发生。此外，我们描述来操纵在斑马鱼神经球的基因表达的步骤。

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Cite this Article

Lopez-Ramirez, M. A., Calvo, C., Ristori, E., Thomas, J., Nicoli, S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (108), e53617, doi:10.3791/53617 (2016).

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