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Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

12,609 Views

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11:01 min

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February 29, 2016

DOI:

10.3791/53617-v

DOI:
10.3791/53617-v

•

11:01 min
February 29, 2016

•

8 Views
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1Yale Cardiovascular Research Center, Internal Medicine,Yale University, 2Department of Medicine,University of California, San Diego, 3APHP Groupe Hospitalier Pitié-Salpètrière,Université Pierre and Marie Curie

Summary

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Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.

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Adult Zebrafish BrainNeurosphere AssayAdult NeurogenesisNeural Stem CellNeural Progenitor CellIn VitroDissectionMicro-dissectionDMEM/F12 Medium

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Lopez-Ramirez, M. A., Calvo, C., Ristori, E., Thomas, J., Nicoli, S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. J. Vis. Exp. (108), e53617, doi:10.3791/53617 (2016).

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