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Quantifier la cytotoxicité de Staphyloccus aureus contre les leucocytes polymorphes humains
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免疫与感染
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JoVE 杂志 免疫与感染
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
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Quantifier la cytotoxicité de Staphyloccus aureus contre les leucocytes polymorphes humains

5,972 Views

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12:27 min

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January 03, 2020

DOI:

10.3791/60681-v

DOI:
10.3791/60681-v

•

12:27 min
January 03, 2020

•

10 Views
, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Summary

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Ce protocole décrit une méthode pour la purification des leucocytes polymorphes du sang humain entier et deux essais distincts qui quantifient la cytotoxicité de Staphylococcus aureus contre ces cellules immunitaires innées importantes.

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Dankoff, J. G., Pallister, K. B., Guerra, F. E., Parks, A. J., Gorham, K., Mastandrea, S., Voyich, J. M., Nygaard, T. K. Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes. J. Vis. Exp. (155), e60681, doi:10.3791/60681 (2020).

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