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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

DOI:
10.3791/58000-v

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10:05 min

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September 30, 2018

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, , , , ,
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

Chapters

  • 00:04Title
  • 01:05Pancreas Isolation
  • 03:04Tissue Digestion and Islet Isolation
  • 04:53Single-cell Lysis and cDNA Amplification
  • 07:32Results: Single-cell RNA-Seq of Mouse Pancreatic Endocrine Cells
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

Tags

Keywords: Single-cell TranscriptomicsPancreatic Endocrine CellsLineage DifferentiationMaturationRegenerationDiabetesPancreatic Endocrine DiseasesCollagenase PerfusionBile DuctMouse Pancreas

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