Journal
/
Entwicklungsbiologie
/
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

10,701 Views

•

10:05 min

•

September 30, 2018

DOI:

10.3791/58000-v

DOI:
10.3791/58000-v

•

10:05 min
September 30, 2018

•

23 Views
, , , , ,
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

Summary

Automatically generated

We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

Explore More Videos

Single-cell TranscriptomicsPancreatic Endocrine CellsLineage DifferentiationMaturationRegenerationDiabetesPancreatic Endocrine DiseasesCollagenase PerfusionBile DuctMouse Pancreas

Read Article

Cite this Article

Li, L., Yu, X., Zhang, Y., Feng, Y., Qiu, W., Xu, C. Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells. J. Vis. Exp. (139), e58000, doi:10.3791/58000 (2018).

Download .ris file

Copy

Share Video

.

Copy