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Análises de célula única Transcriptomic de células endócrinas no pâncreas de rato
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Developmental Biology
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JoVE Journal Developmental Biology
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Análises de célula única Transcriptomic de células endócrinas no pâncreas de rato

português

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10,701 Views

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10:05 min

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September 30, 2018

DOI:

10.3791/58000-v

DOI:
10.3791/58000-v

•

10:05 min
September 30, 2018

•

23 Views
, , , , ,
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

Summary

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Nós descrevemos um método para o isolamento de células endócrinas de pancreases embrionárias, Neonatais e pós-natal, seguidos por sequenciação do ARN de célula única. Esse método permite que as análises do desenvolvimento de linhagem endócrina do pâncreas, heterogeneidade e transcriptomic dinâmica de células.

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Cite this Article

Li, L., Yu, X., Zhang, Y., Feng, Y., Qiu, W., Xu, C. Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells. J. Vis. Exp. (139), e58000, doi:10.3791/58000 (2018).

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