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Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
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发育生物学
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JoVE 杂志 发育生物学
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

11,044 Views

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09:16 min

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December 14, 2015

DOI:

10.3791/53280-v

DOI:
10.3791/53280-v

•

09:16 min
December 14, 2015

•

2 Views
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1Department of Dermatology,UCSD Stem Cell Program, University of California, San Diego, 2Department of Cellular and Molecular Medicine,UCSD Stem Cell Program, University of California, San Diego

Summary

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The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

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Cite this Article

Li, J., Sen, G. L. Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis. J. Vis. Exp. (106), e53280, doi:10.3791/53280 (2015).

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