需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes
11,275 Views
•
08:36 min
•
August 20, 2015
DOI:
Summary
Automatically generated
This manuscript provides a step-by-step procedure for the derivation and maintenance of human keratinocytes from plucked hair and subsequent generation of integration-free human induced pluripotent stem cells (hiPSCs) by episomal vectors.
Read Article
Cite this Article
Hung, S. S., Pébay, A., Wong, R. C. Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. J. Vis. Exp. (102), e53174, doi:10.3791/53174 (2015).
Copy