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发育生物学
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Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
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发育生物学
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JoVE 杂志 发育生物学
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

12,094 Views

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08:52 min

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March 04, 2015

DOI:

10.3791/52353-v

DOI:
10.3791/52353-v

•

08:52 min
March 04, 2015

•

6 Views
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1Department of Physiology,University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine,National Yang-Ming University, 3Department of Education and Research,Taipei City Hospital

Summary

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Here we describe a technique for studying hippocampal postnatal neurogenesis using the organotypic slice culture technique. This method allows for in vitro manipulation of adult neurogenesis and allows for the direct application of pharmacological agents to the cultured hippocampus.

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Organotypic Slice CulturePostnatal NeurogenesisHippocampusDentate GyrusPharmacological ManipulationDeveloping Granule NeuronsMaturation ProcessRodent Brain

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Mosa, A. J., Wang, S., Tan, Y. F., Wojtowicz, J. M. Organotypic Slice Cultures for Studies of Postnatal Neurogenesis. J. Vis. Exp. (97), e52353, doi:10.3791/52353 (2015).

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