Journal
/
发育生物学
/
Generering af genetisk modificerede Organotypiske Skin Cultures Brug devitaliseret Menneskelig Dermis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis

Generering af genetisk modificerede Organotypiske Skin Cultures Brug devitaliseret Menneskelig Dermis

DOI:
10.3791/53280-v

•

09:16 min

•

December 14, 2015

•

,
1Department of Dermatology,UCSD Stem Cell Program, University of California, San Diego, 2Department of Cellular and Molecular Medicine,UCSD Stem Cell Program, University of California, San Diego

Chapters

  • 00:05Title
  • 00:50Preparation of Devitalized Human Dermis
  • 02:21Genetic Modification of Primary Human Keratinocytes
  • 04:33Building an Organotypic Cassette
  • 05:09Organotypic Skin Cultures
  • 07:03Results: Morphology and Gene Expression Analysis of Genetically Modified Human Epidermis
  • 08:28Conclusion

Summary

自动翻译

自动翻译

The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.

Tags

Organotypic Skin CulturesGenetically Modified KeratinocytesDevitalized Human DermisEpidermal DifferentiationStratificationRetrovirusesGene OverexpressionGene KnockdownSkin HomeostasisGene Expression ProfilingImmunostainingChromatin ImmunoprecipitationHigh-throughput Sequencing

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Organotypiske skivekulturer for Studier af Fødselsdepression Neurogenese

Generation af menneskelige fedt-stamceller gennem dedifferentiering af Ældre Adipocytter i Loft Cultures

Transfektion, Selection, og Colony-picking af menneskeskabte pluripotente stamceller talen målrettet med et GFP-genet i AAVS1 Safe Harbor

3D organotypisk co-kultur Model Støtte medullær thymusepitelmonolag celleproliferation, differentiering og Promiskuøs Gene Expression

Generation af Integration-fri Menneskelige induceret pluripotente stamceller Brug Hair-afledte Keratinocytter

Generation af Integration-fri induceret pluripotente stamceller fra humane perifere mononucleære blodceller Brug episomale vektorer

Brug Primære Neurosfære kulturer til at studere Primær Cilia

En ændret Trier Social Stress Test for sårbare mexicanske amerikanske adolescente

Generering af genetisk modificerede mus gennem mikroinjektion af oocytter

Generation og dyrkning af primære humane keratinocytter fra voksen hud

Read Article