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发育生物学
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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

DOI:
10.3791/52485-v

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07:35 min

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March 07, 2015

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, , , , , , ,
1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University

Chapters

  • 00:00Title
  • 00:17Introduction
  • 00:54Methods
  • 06:00Results
  • 06:56Conclusion

Summary

自动翻译

自动翻译

Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

Tags

Adipose Stem CellsMature AdipocytesCeiling CultureDedifferentiationFABP4Cell IsolationCollagenase DigestionCell Culture

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