Journal
/
/
隔离和免疫淋巴细胞和树突状细胞的小鼠Peyer氏斑
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
DOI:

09:49 min

March 17, 2013

, ,

Chapters

  • 00:05Title
  • 01:40Isolation of PP Cells for Flow Cytometry
  • 03:03Antibody Labeling of Cells for Flow Cytometry
  • 03:48Preparation of PP Cryosections
  • 04:49Cryosectioning
  • 05:20Antibody Labeling of Cryosections and Confocal Microscopy Analysis
  • 06:11Results: Immunological Analyses of Mouse Peyer’s Patches
  • 09:23Conclusion

Summary

自动翻译

理解在派伊尔氏淋巴集结的特定的细胞亚群(PPS),初级感应网站肠相关的淋巴组织的免疫功能有越来越大的兴趣。在这里,我们列出了流式细胞仪分析和PP冰冻切片免疫准备的PP单细胞准备的并行协议。

Related Videos

Read Article