需要订阅 JoVE 才能查看此. 登录或开始免费试用。
‘Bioluminescent’ Reporter Phage for the Detection of Category A Bacterial Pathogens
13,285 Views
•
11:31 min
•
July 08, 2011
DOI:
Summary
Automatically generated
A simple method for the identification of priority bacterial pathogens is to use genetically engineered reporter phage. These reporter phage, which are specific to their particular host species, are capable of rapidly transducing a bioluminescent signal response to host cells. Herein, we describe the use of reporter phage for the detection of Yersinia pestis.
Explore More Videos
Bioluminescent Reporter PhageDetectionCategory A Bacterial PathogensYersinia PestisBacillus AnthracisPlagueAnthraxBioweaponCommunicabilityRapid Clinical CourseHigh Mortality RateOutbreak DetectionMethodologiesRapid Detection And DiagnosisPublic Health MeasuresCrisis ManagementRecombinant Reporter PhageCenters For Disease Control And PreventionPhage Lysis AssaysBacterial Pathogens IdentificationPlaques FormationLaboratory-based AssaysBacterial Isolation And Cultivation
Read Article
Cite this Article
Schofield, D. A., Molineux, I. J., Westwater, C. 'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens. J. Vis. Exp. (53), e2740, doi:10.3791/2740 (2011).
Copy