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A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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JoVE 杂志 免疫与感染
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

8,651 Views

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06:55 min

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November 07, 2018

DOI:

10.3791/58200-v

DOI:
10.3791/58200-v

•

06:55 min
November 07, 2018

•

2 Views
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1State Key Laboratory of Diarrhea Disease Detection,Zhuhai International Travel Healthcare Center

Summary

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Salmonella spp./Shigella spp. are common pathogens attributed to diarrhea. Here, we describe a high-throughput platform for the screening of Salmonella spp./Shigella spp. using real-time PCR combined with guided culture.

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SalmonellaShigellaHigh-throughput ScreeningNAATReal-time PCRCulture IdentificationPathogensVibrioStaphylococcusE. ColiPre-enrichmentCentrifugationDNA ExtractionReal-time PCRSelenite Crystal MediumColony SelectionAutomated Microbial IdentificationO-antigen CharacterizationH-antigen Characterization

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Yang, Z., Chen, X., Tu, C., Su, Y., Wang, H. A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.. J. Vis. Exp. (141), e58200, doi:10.3791/58200 (2018).

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