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High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
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November 28, 2016
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High-throughput testing of DNA and RNA based pathogens by nanoscale PCR is described using a syndromic canine and equine respiratory PCR panel.
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Keyword Extraction:High-throughput DetectionRespiratory PathogensAnimal SpecimensNanoscale PCRReal-time PCRDNARNAPathogensOneHealth SurveillanceInfectious DiseasesClinical SpecimensRespiratory PathogensHorsesDogsLysis BufferReverse TranscriptionPre-amplificationPreamp96-well PlateThermocyclerCDNA Production
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Goodman, L. B., Anderson, R. R., Slater, M., Ortenberg, E., Renshaw, R. W., Chilson, B. D., Laverack, M. A., Beeby, J. S., Dubovi, E. J., Glaser, A. L. High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR. J. Vis. Exp. (117), e54781, doi:10.3791/54781 (2016).
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