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Introducing Shear Stress in the Study of Bacterial Adhesion
Chapters
- 00:05Title
- 01:32Human Host Cell and Bacterial Culture
- 03:21Initial Adhesion of Individual Bacteria to Host Cells
- 06:41Quantification of Initial Adhesion of Individual Bacteria to Host Cells
- 07:50Measuring the Resistance to Flow of Individual Bacteria Adherent to Host Cells
- 08:23Measuring the Growth of an Isolated Adherent Bacterium to a Microcolony
- 09:28Measuring the Resistance to Flow of Adherent Bacterial Microcolonies
- 11:14Bacterial Detachment from Microcolonies
- 11:57Bacterial Adhesion and Attachment: Representative Data
- 12:49Conclusion
During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.
