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Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
Chapters
- 00:04Title
- 01:21Culture, Differentiation, and Validation of HL-60 Cells
- 02:30Preparation of Bacterial Stock Samples
- 03:53Bacterial Treatment and Culture
- 04:43HL-60 Bacterial Co-culture
- 05:46Sample Plating and Overnight Incubation
- 06:55Results: Determining Bacterial Cell Viability using OPKA
- 07:55Conclusion
This opsonophagocytic killing assay is used to compare the ability of phagocytic immune cells to respond to and kill bacteria based on different treatments and/or conditions. Classically, this assay serves as the gold standard for assessing effector functions of antibodies raised against a bacterium as opsonin.
