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Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays
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Desenvolvimento e aplicação de ensaios biofísicos para avaliação da formação de complexos ternários induzidos por proteólise visando quimeras (PROTACS)
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Desenvolvimento e aplicação de ensaios biofísicos para avaliação da formação de complexos ternários induzidos por proteólise visando quimeras (PROTACS)

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1,835 Views

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01:09 min

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January 12, 2024

DOI:

10.3791/65718-v

DOI:
10.3791/65718-v

•

01:09 min
January 12, 2024

•

41 Views
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1Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

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Biophysical AssaysTernary Complex FormationProteolysis Targeting Chimeras (PROTACs)Surface Plasmon Resonance (SPR)Biolayer Interferometry (BLI)Isothermal Titration Calorimetry (ITC)Brd4BD2VHLPPM1DCereblon (CRBN)KineticsThermodynamicsCooperativityHook Effect

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Jiang, W., Soutter, H. The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS). J. Vis. Exp. (203), e65718, doi:10.3791/65718 (2024).

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