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Developmental Biology
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通过鸡胚胎的腹内移植对肾脏类器官进行有效的血管化
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Developmental Biology
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JoVE Journal
Developmental Biology
Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
Please note that all translations are automatically generated.
Click here for the English version.
通过鸡胚胎的腹内移植对肾脏类器官进行有效的血管化
DOI:
10.3791/65090-v
•
07:35 min
•
February 17, 2023
•
Marije Koning
2
,
Ellen Lievers
2
,
Thierry Jaffredo
,
Cathelijne W. van den Berg
2,4
,
Ton J. Rabelink
2,4
1
Department of Internal Medicine – Nephrology
,
Leiden University Medical Center
,
2
Einthoven Laboratory of Vascular and Regenerative Medicine
,
Leiden University Medical Center
,
3
IBPS, CNRS UMR7622, Developmental Biology Laboratory
,
Sorbonne Université
,
4
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
,
Leiden University Medical Center
Chapters
00:04
Introduction
00:30
Preparing Chicken Embryos for Transplantation
02:14
Intracelomic Transplantation on Day 4 of Incubation
03:18
Transplantation
04:09
Injection
04:59
Collecting Transplanted Organoids on Day 12 of Incubation
06:12
Results: Analysing the Transplanted Kidney Organoids
07:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了在鸡胚胎的天体腔中移植肾脏类器官的详细方案。该方法在8天内诱导类器官的血管形成和增强成熟,可用于以有效的方式研究这些过程。
Tags
Kidney Organoids
Vascularization
Intracoelomic Transplantation
Chicken Embryos
In Vitro Maturation
HIPSC-derived
Egg Incubation
Embryo Visualization
Coelom Access
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