Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Efficient Vascularization of Kidney Organoids…
JoVE Journal
Developmental Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Developmental Biology
Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
 

Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

Article DOI: 10.3791/65090-v • 07:35 min • February 17th, 2023
February 17th, 2023 •
1,2, 1,2, 3, 1,2,4, 1,2,4
1Department of Internal Medicine - Nephrology, Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory, Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center

Chapitres

résumé
Automatic Translation

Here, we present a detailed protocol for the transplantation of kidney organoids in the celomic cavity of chicken embryos. This method induces vascularization and enhanced maturation of the organoids within 8 days and can be used to study these processes in an efficient manner.

Tags

Efficient Vascularization Kidney Organoids Intracoelomic Transplantation Chicken Embryos In Vitro Immature Applicability Maturation Procedure Transplantation Analysis HIPSC-derived Kidney Organoids Fertilized Leghorn Eggs Window Eggshell Transparent Tape Hole 19-gauge Needle Five-milliliter Syringe Albumin Embryo Conceal Pencil Marked Upward Facing Side Circular Window Curved Dissecting Scissors
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter