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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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07:35 min
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February 17, 2023
DOI:
1Department of Internal Medicine – Nephrology,Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine,Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory,Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden University Medical Center
Summary
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Here, we present a detailed protocol for the transplantation of kidney organoids in the celomic cavity of chicken embryos. This method induces vascularization and enhanced maturation of the organoids within 8 days and can be used to study these processes in an efficient manner.
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Koning, M., Lievers, E., Jaffredo, T., van den Berg, C. W., Rabelink, T. J. Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos. J. Vis. Exp. (192), e65090, doi:10.3791/65090 (2023).
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