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AMEBaS : Extraction automatique de la ligne médiane et soustraction de l’arrière-plan des time-lapses de fluorescence ratiométrique de cellules uniques polarisées
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JoVE Journal Developmental Biology
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
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AMEBaS : Extraction automatique de la ligne médiane et soustraction de l’arrière-plan des time-lapses de fluorescence ratiométrique de cellules uniques polarisées

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326 Views

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06:03 min

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June 23, 2023

DOI:

10.3791/64857-v

DOI:
10.3791/64857-v

•

06:03 min
June 23, 2023

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1 Views
, , , , , , ,
1Institute of Mathematics and Statistics,University of São Paulo, 2Center for Mathematics, Computing and Cognition,Federal University of ABC, 3Department of Botany, Institute of Biosciences,University of São Paulo, 4Cell Biology and Molecular Genetics Department,University of Maryland, 5Department of Biosciences,University of Milan, 6Department of Physics,Politecnico di Milano

Summary

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Les méthodes actuelles d’analyse de la dynamique intracellulaire des cellules uniques polarisées sont souvent manuelles et manquent de standardisation. Ce manuscrit présente un nouveau pipeline d’analyse d’images pour automatiser l’extraction médiane de cellules polarisées uniques et quantifier le comportement spatio-temporel à partir de timelapses dans une interface en ligne conviviale.

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AMEBaSMidline ExtractionBackground SubtractionRatiometric FluorescenceTime-lapsePolarized Single CellsAutomationCell SegmentationCell Midline TracingSkeletonizationLinear ExtrapolationInterpolation

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Cite this Article

Badain, R., Damineli, D. S. C., Portes, M. T., Feijó, J., Buratti, S., Tortora, G., Neves de Oliveira, H., Cesar Jr, R. M. AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells. J. Vis. Exp. (196), e64857, doi:10.3791/64857 (2023).

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