/
/
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
A subscription to JoVE is required to view this content. Sign in or start your free trial.
326 Views
•
06:03 min
•
June 23, 2023
DOI:
1Institute of Mathematics and Statistics,University of São Paulo, 2Center for Mathematics, Computing and Cognition,Federal University of ABC, 3Department of Botany, Institute of Biosciences,University of São Paulo, 4Cell Biology and Molecular Genetics Department,University of Maryland, 5Department of Biosciences,University of Milan, 6Department of Physics,Politecnico di Milano
Summary
Automatically generated
Current methods for analyzing the intracellular dynamics of polarized single cells are often manual and lack standardization. This manuscript introduces a novel image analysis pipeline for automating midline extraction of single polarized cells and quantifying spatiotemporal behavior from time lapses in a user-friendly online interface.
Read Article
Cite this Article
Badain, R., Damineli, D. S. C., Portes, M. T., Feijó, J., Buratti, S., Tortora, G., Neves de Oliveira, H., Cesar Jr, R. M. AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells. J. Vis. Exp. (196), e64857, doi:10.3791/64857 (2023).
Copy