Journal
/
Developmental Biology
/
Differentiering af humane pluripotente stamceller i insulinproducerende øklynger
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differentiering af humane pluripotente stamceller i insulinproducerende øklynger

DOI:
10.3791/64840-v

•

08:41 min

•

June 23, 2023

•

, , , , , ,
1Department of Cellular & Physiological Sciences, Life Science Institute,University of British Columbia, 2School of Biomedical Engineering,University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery,University of British Columbia

Chapters

  • 00:00Introduction
  • 00:35Differentiation of hPSCs into Pancreatic Progenitor Cells
  • 02:48Aggregation into Pancreatic Progenitor Clusters Using 400 μm Diameter Microwell Plates
  • 04:20Differentiation of Kit-Derived Pancreatic Progenitor Clusters into hPSC-Islets
  • 06:07Results: Generation of hPSC-Islets Capable of Secreting Insulin upon Glucose Challenge
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Differentieringen af stamceller i øceller giver en alternativ løsning til konventionel diabetesbehandling og sygdomsmodellering. Vi beskriver en detaljeret stamcellekulturprotokol, der kombinerer et kommercielt differentieringssæt med en tidligere valideret metode til at hjælpe med at producere insulinudskillende, stamcelleafledte øer i en skål.

Tags

Human Pluripotent Stem CellsInsulin-producing Islet ClustersStatic Suspension CulturePancreatic ProgenitorsIslet-like ClustersHESC-qualified MatrigelCell DissociationStem Cell CultureStage 1A MediumStage 1B MediumStage 2A MediumStage 2B MediumStage 3 MediumStage 4 MediumAnti-adherence Rinsing SolutionMicrowell Plate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Stencil Micropatterning for Human pluripotente stamceller til Sondering Spatial Organisation af differentiering Fates

Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller

In vitro differentiering af humane pluripotente stamceller til trofoblastceller

Protokol for differentieringen af ​​humane inducerede pluripotente stamceller i blandede kulturer af neuroner og glia til neurotoksicitetstestning

In Vitro Differentiering af humane mesenkymale stamceller i funktionel Cardiomyocyte-lignende celler

Hurtig, instrueret differentiering af retinale Pigment epitel celler fra menneskelige embryonale eller induceret pluripotente stamceller

Effektiv og skalerbar styret differentiering af klinisk kompatibel hornhinde Limbal epitelial stamceller fra humane pluripotente stamceller

RNA-baserede omprogrammering af menneskelige primære fibroblaster til induceret pluripotente stamceller

Effektiv differentiering af humane pluripotente stamceller i leverceller

I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller

Read Article