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Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
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Chapters
- 00:05Title
- 01:02Preparing the Stencil for Cell Seeding
- 02:32Seeding Human Embryonic Stem Cells onto the Stenciled Substrate
- 04:39Stencil Removal and Passivation of Unpatterned Substrate
- 06:20Results: Mechanically Patterning Early Mesoendermal Differentiation
- 07:39Conclusion
Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.
