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Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

1,801 Views

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08:41 min

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June 23, 2023

DOI:

10.3791/64840-v

DOI:
10.3791/64840-v

•

08:41 min
June 23, 2023

•

48 Views
, , , , , ,
1Department of Cellular & Physiological Sciences, Life Science Institute,University of British Columbia, 2School of Biomedical Engineering,University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery,University of British Columbia

Summary

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The differentiation of stem cells into islet cells provides an alternative solution to conventional diabetes treatment and disease modeling. We describe a detailed stem cell culture protocol that combines a commercial differentiation kit with a previously validated method to aid in producing insulin-secreting, stem cell-derived islets in a dish.

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Cite this Article

Zhao, J., Liang, S., Braam, M. J. S., Baker, R. K., Iworima, D. G., Quiskamp, N., Kieffer, T. J. Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters. J. Vis. Exp. (196), e64840, doi:10.3791/64840 (2023).

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