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ヒト多能性幹細胞のインスリン産生膵島クラスターへの分化
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters
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ヒト多能性幹細胞のインスリン産生膵島クラスターへの分化

日本語

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1,804 Views

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08:41 min

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June 23, 2023

DOI:

10.3791/64840-v

DOI:
10.3791/64840-v

•

08:41 min
June 23, 2023

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48 Views
, , , , , ,
1Department of Cellular & Physiological Sciences, Life Science Institute,University of British Columbia, 2School of Biomedical Engineering,University of British Columbia, 3STEMCELL Technologies Inc, 4Department of Surgery,University of British Columbia

Summary

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幹細胞の膵島細胞への分化は、従来の糖尿病治療や疾患モデリングに代わるソリューションを提供します。市販の分化キットと以前に検証された方法を組み合わせて、インスリン分泌性幹細胞由来の膵島を皿で産生するのに役立つ詳細な幹細胞培養プロトコルについて説明します。

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Cite this Article

Zhao, J., Liang, S., Braam, M. J. S., Baker, R. K., Iworima, D. G., Quiskamp, N., Kieffer, T. J. Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters. J. Vis. Exp. (196), e64840, doi:10.3791/64840 (2023).

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