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Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope
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Neuroscience
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JoVE Journal Neuroscience
Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

English

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853 Views

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08:25 min

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April 21, 2023

DOI:

10.3791/64701-v

DOI:
10.3791/64701-v

•

08:25 min
April 21, 2023

•

3 Views
, , , ,
1Department of Neurology,University of Massachusetts Chan Medical School, 2Department of Neurosurgery,The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology,University of Massachusetts Chan Medical School

Summary

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This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.

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Cite this Article

Zhong, J., Gunner, G., Henninger, N., Schafer, D. P., Bosco, D. A. Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope. J. Vis. Exp. (194), e64701, doi:10.3791/64701 (2023).

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